Quilmes National University

Long-term 3D cultures typically end with poor results when performed under static conditions. Thus, one critical aspect is to obtain adequate distributions of nutrients and oxygen across the whole volume of the sample. The improvement of mass transport and nutrient exchange in three-dimensional in-vitro experiments is crucial to get viable cells populations, for which the imposing of certain flow rate the culture media across the scaffold (perfusion) has been experimentally shown very efficacious compared to static cultures.